Tg(Camk2a-rtTA)1237Kndl
Transgene Detail

Summary

• Symbol: Tg(Camk2a-rtTA)1237Kndl
• Name: transgene insertion 1237, Eric R Kandel
• MGI ID: MGI:3716991
• Synonyms: CamKIIalpha promoter-rtTA transgene, CamKII-rtTA2, line 1237
• Transgene: Tg(Camk2a-rtTA)1237Kndl Location: unknown
• Alliance: Tg(Camk2a-rtTA)1237Kndl page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: The transgene comprises 8.5 kb of upstream flanking DNA and 85 bp of the 5', noncoding exon of the mouse calcium/calmodulin-dependent protein kinase II alpha gene, an artificial intron and splice signal, the coding sequence for the reverse tetracycline-regulated transactivator (rtTA) protein and an SV40 polyadenylation signal. Maximal doxycycline induction of mice bearing both this transgene and a lacZ reporter transgene under control of the tetracycline operator (tetO) demonstrated expression in the CA1 and CA2 regions, but only minimal expression in the CA3 region, of the hippocampus; in the dentate gyrus; in both superficial layers and a deep layer of the cortex; and in the septum and striatum. (J:123059)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two transgenic lines, designated 1237 and 1076, were developed. Data were reported only for the former; authors state that results for line 1076 are similar to those for line 1237.

References

• Original: J:123059 Mansuy IM, et al., Inducible and reversible gene expression with the rtTA system for the study of memory. Neuron. 1998 Aug;21(2):257-65
• All: 13 reference(s)