Cd79b^tm168Mnz
Targeted Allele Detail

Summary

• Symbol: Cd79b^tm168Mnz
• Name: CD79B antigen; targeted mutation 168, Michel C Nussenzweig
• MGI ID: MGI:3720567
• Synonyms: IgBISceI
• Gene: Cd79b Location: Chr11:106202167-106205388 bp, - strand Genetic Position: Chr11, 68.89 cM
• Alliance: Cd79b^tm168Mnz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148011
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: A targeting vector containing a self-excising lox-P flanked neomycin resistance cassette was used to introduce an 18 bp I-SceI (S. cerevisiae) endonuclease recognition site (TAGGGATAACAGGGTAAT) in intron 2 of the gene. The construct was electroporated into CY2.4 albino C57BL/6J-Tyr^c-2J-derived embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male chimeric animals were crossed to C57BL/6J-Tyr^c-2J mice to allow for coat-color screening of germline transmission. White pups carrying the mutation were then crossed to C57BL/6 background EIIA Cre mice to excise the neomycin cassette, leaving one loxP site in intron three of the gene. Crosses with C57BL/6 were carried out to remove the Cre transgene. (J:148011)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd79b Mutation: 36 strains or lines available

References

• Original: J:148011 Nussenzweig M, Generation of a knock-in allele of Cd79b. MGI Direct Data Submission. 2009
• All: 1 reference(s)