Nlrp3^tm1Tsc
Targeted Allele Detail

Summary

• Symbol: Nlrp3^tm1Tsc
• Name: NLR family, pyrin domain containing 3; targeted mutation 1, Jurg Tschopp
• MGI ID: MGI:3721141
• Synonyms: NALP3-
• Gene: Nlrp3 Location: Chr11:59432395-59457781 bp, + strand Genetic Position: Chr11, 37.73 cM, cytoband B1.3
• Alliance: Nlrp3^tm1Tsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124226
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The gene was disrupted by the insertion of an EGFP cassette fused in frame with the ATG of exon 2. A selection cassette PGK-neo flanked by 2 loxP sites was also inserted in the intron 2. The neo cassette was deleted by backcrossing the mice with a Cre-expressing deleter strain. RT-PCR analysis of RNA isolated from mutant macrophages confirmed that the wild-type transcript is absent. (J:124226)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nlrp3 Mutation: 64 strains or lines available

References

• Original: J:124226 Martinon F, et al., Gout-associated uric acid crystals activate the NALP3 inflammasome. Nature. 2006 Mar 9;440(7081):237-41
• All: 139 reference(s)