Mrc2^tm1Cmi
Targeted Allele Detail

Summary

• Symbol: Mrc2^tm1Cmi
• Name: mannose receptor, C type 2; targeted mutation 1, Clare M Isacke
• MGI ID: MGI:3722867
• Synonyms: Endo180^deltaEx2-6, Endo180deltaEx2-6
• Gene: Mrc2 Location: Chr11:105183469-105241965 bp, + strand Genetic Position: Chr11, 68.73 cM, cytoband E1
• Alliance: Mrc2^tm1Cmi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124909
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A targeting vector was designed to replace exons 2-5 and part of exon 6 with a neomycin resistance cassette. Analysis of mutant MEFs determined that a transcript is produced, and sequencing showed it resulted from exon 1 being spliced to exon 7, with a continuous open reading frame. A protein lacking the cysteine-rich domain (exon 2), the FNII domain (exon 3) and the first CTLD (exons 4-6) was expressed. (J:124909)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Mrc2 Mutation: 120 strains or lines available

References

• Original: J:124909 East L, et al., A targeted deletion in the endocytic receptor gene Endo180 results in a defect in collagen uptake. EMBO Rep. 2003 Jul;4(7):710-6
• All: 4 reference(s)