Tg(CAG-Serpine1)1Dgi
Transgene Detail

Summary

• Symbol: Tg(CAG-Serpine1)1Dgi
• Name: transgene insertion 1, David Ginsburg
• MGI ID: MGI:3763900
• Synonyms: PAI-1 line 1, PAI1 transgenic (actin promoter), Tg(Actb-Serpine1)1Dgi
• Transgene: Tg(CAG-Serpine1)1Dgi Location: unknown
• Alliance: Tg(CAG-Serpine1)1Dgi page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAG-Serpine1)1Dgi expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Serpine1 (MGI:97608)

• Mutation details: Oligo-based site-directed mutagenesis was use to mutate codon 177 from AAG to ACC (K154T), and codon 342 from CAG to CTG (Q319L) in the mature mouse protein. Expression is driven by the CAG promoter (the chicken beta-actin (Actb) promoter with a CMV immediate-early enhancer). The 2 amino acid substitutions more than triple the functional half-life of the protein and expression is increased approximately 170-fold over wildtype. Quantitative PCR reveals elevated expression in brain, heart, lung, liver, spleen, intestine, kidney, fat, skin, and muscle (strongest in liver, brain, heart and kidney). Immunohistochemistry reveals expression in kidney, liver, spleen, and lung. (J:167969)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:167969 Fahim AT, et al., Transgenic overexpression of a stable Plasminogen Activator Inhibitor-1 variant. Thromb Res. 2009 Mar;123(5):785-92
• All: 3 reference(s)