Stat1^tm1Tdec
Targeted Allele Detail

Summary

• Symbol: Stat1^tm1Tdec
• Name: signal transducer and activator of transcription 1; targeted mutation 1, Thomas Decker
• MGI ID: MGI:3774319
• Synonyms: Stat1^S272A, Stat1^tm1(S727A)
• Gene: Stat1 Location: Chr1:52158599-52201024 bp, + strand Genetic Position: Chr1, 26.81 cM
• Alliance: Stat1^tm1Tdec page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130622
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 22 and 23a were replaced with a segment containing exon 22, a floxed neo cassette and exon 23 containing a nucleotide substitution that results in an amino acid substitution of an alanine for a serine at position 727 (S727A), a site within the transactivation domain. The neo cassette was removed by germ-line, cre-mediated recombination. Protein expression and the absence of phosphorylation was confirmed by western blot analysis. (J:130622)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stat1 Mutation: 74 strains or lines available

References

• Original: J:130622 Varinou L, et al., Phosphorylation of the Stat1 transactivation domain is required for full-fledged IFN-gamma-dependent innate immunity. Immunity. 2003 Dec;19(6):793-802
• All: 7 reference(s)