Hras^tm2Xbr
Targeted Allele Detail

Summary

• Symbol: Hras^tm2Xbr
• Name: Harvey rat sarcoma virus oncogene; targeted mutation 2, Xose R Bustelo
• MGI ID: MGI:3803587
• Synonyms: H-Ras^G12V
• Gene: Hras Location: Chr7:140770839-140773938 bp, - strand Genetic Position: Chr7, 86.48 cM
• Alliance: Hras^tm2Xbr page

Virgin Hras^tm2Xbr/Hras^tm2Xbr females display mammary gland hyperplasia

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137729
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Single point mutation
• Mutation details: In ES cells containing Hras1^tm1Xbr, a floxed hygro STOP cassette was inserted upstream of coding exon 1. In addition, a G to T substitutions was created at the second base of codon 12 of exon 1. This results in an amino acid substitution of valine for glycine. Germ line, cre-mediated recombination was used to remove the hygro STOP cassette. The final allele contains the G12V mutation and the IRES-beta-geo cassette. (J:137729)

Disease models

Expression

In Structures Affected by this Mutation:

19 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hras Mutation: 30 strains or lines available

Notes

This mutation was generated by re-targeting R1 ES cells in which Hras had been targeted to create Hras^tm1Xbr. (J:137729)

References

• Original: J:137729 Schuhmacher AJ, et al., A mouse model for Costello syndrome reveals an Ang II-mediated hypertensive condition. J Clin Invest. 2008 Jun;118(6):2169-79
• All: 8 reference(s)