Muc16^tm1Bhr
Targeted Allele Detail

Summary

• Symbol: Muc16^tm1Bhr
• Name: mucin 16; targeted mutation 1, Richard R Behringer
• MGI ID: MGI:3840843
• Gene: Muc16 Location: Chr9:18406751-18585826 bp, - strand Genetic Position: Chr9, 7.06 cM, cytoband A3
• Alliance: Muc16^tm1Bhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147379
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A 6 kb fragment including most of exon 3 and part of intron 3 was replaced with an IRES-T-lacZpA-loxP-PGKneobpA- loxP reporter cassette via homologous recombination. No lacZ expression was detected. RT-PCR confirmed the absence of transcripts containing exon 3 but detected alternatively spliced transcripts in the testis of homozygous mice. RT-PCR also confirmed the absence of all expression in the lung, ovary and oviduct from homozygous mice. Immunofluorescence did not detect protein expression in the testis from homozygous mice. (J:147379)

Generation of the Muc16^tm1Bhr allele

Tumor Data

List all tumor models in MMHCdb carrying Muc16^tm1Bhr

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Muc16 Mutation: 207 strains or lines available

References

• Original: J:147379 Cheon DJ, et al., CA125/MUC16 is dispensable for mouse development and reproduction. PLoS One. 2009;4(3):e4675
• All: 5 reference(s)