Ube3b^{Gt(RRJ142)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Ube3b^{Gt(RRJ142)Byg}
• Name: ubiquitin protein ligase E3B; gene trap RRJ142, BayGenomics
• MGI ID: MGI:4129255
• Gene: Ube3b Location: Chr5:114518668-114559230 bp, + strand Genetic Position: Chr5, 55.99 cM
• Alliance: Ube3b^{Gt(RRJ142)Byg} page

Mutation origin

• Mutant Cell Line: RRJ142
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted between exon 19 and 20. Quantitative RT-PCR confirmed the absence of transcript expression. (J:203652)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRJ142	CC326178 ; (MGI Seq Detail)	5' RACE	Chr5:114542735-114546274 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  6 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ube3b Mutation: 70 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)