Tg(IgkH220-17)1Mwg
Transgene Detail

Summary

• Symbol: Tg(IgkH220-17)1Mwg
• Name: transgene insertion 1, Martin Weigert
• MGI ID: MGI:4357863
• Synonyms: Tg(IgK-V8, IgK-J5)1Tnm, Vkappa8 Tg
• Transgene: Tg(IgkH220-17)1Mwg Location: unknown
• Alliance: Tg(IgkH220-17)1Mwg page

Transgene origin

• Strain of Origin: (C57BL/6JNIcr x SJL/JIcr)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(IgkH220-17)1Mwg expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Igk (MGI:96494)

• Mutation details: The transgene comprises the rearranged immunoglobulin kappa light chain (Igk) gene, combining an Igk-V8 and the Igk-J5 segment, of the hybridoma cell line H220-17. It encodes the light chain of most primary antibodies generated in BALB/c mice against the antigenic site, Sb, of the hemagglutinin (HA) of influenza virus A/PR/8/34 (PR8), for which H220-17 is specific. Transgene derived transcripts are detected by northern blot analysis in spleen, but not in liver, and none were detected by dot blot analysis in any non-lymphoid tissue tested. (J:121575)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Three transgenic mouse lines, designated L, Ga and Gb, were derived from two founder animals, one having two integrations (J:121575). The line(s) employed in later studies are not specified.

In combination with the immunoglobulin heavy chain gene encoded by Tg(Igh3H9)41Mwg, the light chain produced from this transgene forms an antibody with specificity for single stranded (ss) DNA (J:119625).

References

• Original: J:121575 Carmack CE, et al., Influence of a V kappa 8 L chain transgene on endogenous rearrangements and the immune response to the HA(Sb) determinant on influenza virus. J Immunol. 1991 Sep 15;147(6):2024-33
• All: 6 reference(s)