Zfp184^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zfp184^{tm1a(KOMP)Wtsi}
• Name: zinc finger protein 184 (Kruppel-like); targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362182
• Gene: Zfp184 Location: Chr13:22129264-22144949 bp, + strand Genetic Position: Chr13, 8.08 cM
• Alliance: Zfp184^{tm1a(KOMP)Wtsi} page
• IMPC: Zfp184 gene page

Mutation origin

• Mutant Cell Lines: EPD0016_3_A03, EPD0016_3_B03, EPD0016_3_C01, EPD0016_3_D02, EPD0016_3_E01, EPD0016_3_F01, EPD0016_3_F02, EPD0016_3_F03, EPD0016_3_G01, EPD0016_3_H03, EPD0017_3_A07, EPD0017_3_A08, EPD0017_3_B07, EPD0017_3_B08, EPD0017_3_C09, EPD0017_3_D08, EPD0017_3_D09, EPD0017_3_E07, EPD0017_3_E09, EPD0017_3_F07, EPD0017_3_F08, EPD0017_3_F09, EPD0017_3_H07, EPD0017_3_H09
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_st1
• Mutation details: The L1L2_st1 cassette was inserted at position 22130749 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 22131504. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Zfp184 Mutation: 40 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)