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Polr1etm1a(KOMP)Wtsi
Targeted Allele Detail
Summary
Symbol: Polr1etm1a(KOMP)Wtsi
Name: polymerase (RNA) I polypeptide E; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4362193
Gene: Polr1e  Location: Chr4:45018609-45034279 bp, + strand  Genetic Position: Chr4, 23.67 cM, cytoband B2
Alliance: Polr1etm1a(KOMP)Wtsi page
IMPC: Polr1e gene page
Mutation
origin
Mutant Cell Lines:  EPD0032_2_A06, EPD0032_2_A08, EPD0032_2_B06, EPD0032_2_B07, EPD0032_2_B08, EPD0032_2_C06, EPD0032_2_C07, EPD0032_2_D06, EPD0032_2_D07, EPD0032_2_D08, EPD0032_2_E06, EPD0032_2_E08, EPD0032_2_F07, EPD0032_2_F08, EPD0032_2_G07, EPD0032_2_G08, EPD0032_2_H06, EPD0032_2_H07
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 45021767 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 45022546. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 4 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 1 line available
Carrying any Polr1e Mutation:  27 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory