Becn1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Becn1^{tm1a(KOMP)Wtsi}
• Name: beclin 1, autophagy related; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362198
• Gene: Becn1 Location: Chr11:101179084-101193112 bp, - strand Genetic Position: Chr11, 64.58 cM, cytoband D
• Alliance: Becn1^{tm1a(KOMP)Wtsi} page
• IMPC: Becn1 gene page

Mutation origin

• Mutant Cell Lines: EPD0168_1_A12, EPD0168_1_B09, EPD0168_1_B11, EPD0168_1_C12, EPD0168_1_D09, EPD0168_1_D11, EPD0168_1_E09, EPD0168_1_E12, EPD0168_1_F09, EPD0168_1_F10, EPD0168_1_F11, EPD0168_1_F12, EPD0168_1_G09, EPD0168_1_G10, EPD0168_1_G11, EPD0168_1_G12, EPD0168_1_H10, EPD0168_1_H11
• Germline Transmission: Earliest citation of germline transmission: J:272251
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 101185572 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 101187632. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Mice Carrying this Mutation:

26 assay results

Tumor Data

List all tumor models in MMHCdb carrying Becn1^{tm1a(KOMP)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Becn1 Mutation: 36 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 13 reference(s)