Kifap3^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kifap3^{tm1a(KOMP)Wtsi}
• Name: kinesin-associated protein 3; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362297
• Gene: Kifap3 Location: Chr1:163607152-163744678 bp, + strand Genetic Position: Chr1, 71.08 cM, cytoband H1
• Alliance: Kifap3^{tm1a(KOMP)Wtsi} page
• IMPC: Kifap3 gene page

Mutation origin

• Mutant Cell Lines: EPD0320_1_A08, EPD0320_1_A09, EPD0320_1_A11, EPD0320_1_B09, EPD0320_1_B10, EPD0320_1_C08, EPD0320_1_C09, EPD0320_1_D08, EPD0320_1_E08, EPD0320_1_E10, EPD0320_1_F08, EPD0320_1_F09, EPD0320_1_F10, EPD0320_1_G08, EPD0320_1_H08, EPD0320_1_H10
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 163623126 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 163623854. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Kifap3 Mutation: 53 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 6 reference(s)