Iftap^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Iftap^{tm1a(KOMP)Wtsi}
• Name: intraflagellar transport associated protein; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362302
• Synonyms: B230118H07Rik^{tm1a(KOMP)Wtsi}, NWC-KOMP
• Gene: Iftap Location: Chr2:101391124-101459376 bp, - strand Genetic Position: Chr2, 53.83 cM, cytoband E2
• Alliance: Iftap^{tm1a(KOMP)Wtsi} page
• IMPC: Iftap gene page

Mutation origin

• Mutant Cell Lines: EPD0067_1_A05, EPD0067_1_B06, EPD0067_1_C04, EPD0067_1_C06, EPD0067_1_D04, EPD0067_1_D05, EPD0067_1_E04, EPD0067_1_E05, EPD0067_1_G04, EPD0067_1_G06, EPD0067_1_H04
• Germline Transmission: Earliest citation of germline transmission: J:236765
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 101413619 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 101414384. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 2 lines available
• Carrying any Iftap Mutation: 21 strains or lines available

Notes

Special note about mutant cell line EPD0067_1_B06 used in J:236765: The mutation generated in this ES cell line results in a hypomorphic allele. RT-PCR analysis revealed that synthesis of full-length transcript is strongly suppressed but not completely abrogated in homozygous mutant testes.

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)