Uck1^{tm2a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Uck1^{tm2a(KOMP)Wtsi}
• Name: uridine-cytidine kinase 1; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362314
• Gene: Uck1 Location: Chr2:32145014-32150117 bp, - strand Genetic Position: Chr2, 22.09 cM
• Alliance: Uck1^{tm2a(KOMP)Wtsi} page
• IMPC: Uck1 gene page

Mutation origin

• Mutant Cell Lines: EPD0048_1_A02, EPD0048_1_B03, EPD0048_1_C01, EPD0048_1_C02, EPD0048_1_C03, EPD0048_1_D02, EPD0048_1_D03, EPD0048_1_E01, EPD0048_1_E02, EPD0048_1_E03, EPD0048_1_F01, EPD0048_1_F02, EPD0048_1_F03, EPD0048_1_G02, EPD0048_1_H02
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 32147881 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 32149134. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Uck1 Mutation: 33 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)