Prrt2^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Prrt2^{tm1a(KOMP)Wtsi}
• Name: proline-rich transmembrane protein 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362325
• Synonyms: Prrt2^fl, PRRT2 KO
• Gene: Prrt2 Location: Chr7:126616703-126620383 bp, - strand Genetic Position: Chr7, 69.28 cM
• Alliance: Prrt2^{tm1a(KOMP)Wtsi} page
• IMPC: Prrt2 gene page

Mutation origin

• Mutant Cell Lines: EPD0284_6_A06, EPD0284_6_A08, EPD0284_6_B05, EPD0284_6_B07, EPD0284_6_B08, EPD0284_6_C07, EPD0284_6_C08, EPD0284_6_D06, EPD0284_6_D08, EPD0284_6_E06, EPD0284_6_E07, EPD0284_6_E08, EPD0284_6_F05, EPD0284_6_F06, EPD0284_6_F07, EPD0284_6_F08, EPD0284_6_G06, EPD0284_6_G08, EPD0284_6_H06, EPD0284_6_H07, EPD0284_6_H08
• Germline Transmission: Earliest citation of germline transmission: J:211773
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 126618446 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 126619783. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:237823)

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 1 line available
• Carrying any Prrt2 Mutation: 17 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 15 reference(s)