Melk^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Melk^{tm1a(KOMP)Wtsi}
• Name: maternal embryonic leucine zipper kinase; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362376
• Gene: Melk Location: Chr4:44300876-44364301 bp, + strand Genetic Position: Chr4, 23.46 cM
• Alliance: Melk^{tm1a(KOMP)Wtsi} page
• IMPC: Melk gene page

Mutation origin

• Mutant Cell Lines: EPD0033_3_A02, EPD0033_3_B02, EPD0033_3_C02, EPD0033_3_D01, EPD0033_3_E02, EPD0033_3_F01, EPD0033_3_F02, EPD0033_3_G02
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 44305611 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 44306427. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:228548)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Melk Mutation: 41 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)