Ogfod1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ogfod1^{tm1a(KOMP)Wtsi}
• Name: 2-oxoglutarate and iron-dependent oxygenase domain containing 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362420
• Gene: Ogfod1 Location: Chr8:94763826-94794549 bp, + strand Genetic Position: Chr8, 46.08 cM
• Alliance: Ogfod1^{tm1a(KOMP)Wtsi} page
• IMPC: Ogfod1 gene page

Mutation origin

• Mutant Cell Lines: EPD0064_2_A12, EPD0064_2_B12, EPD0064_2_C10, EPD0064_2_C11, EPD0064_2_C12, EPD0064_2_D10, EPD0064_2_G10, EPD0064_2_G12, EPD0064_2_H11, EPD0064_2_H12
• Germline Transmission: Earliest citation of germline transmission: J:207410
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 94778264 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 94779080. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 2 lines available
• Carrying any Ogfod1 Mutation: 31 strains or lines available

Notes

Cell lines EPD0064_2_B12 and EPD0064_2_C11 were successfully used to make chimeric mice. Germline transmission was accomplished for both lines. J:207410

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)