Gatc^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gatc^{tm1a(KOMP)Wtsi}
• Name: glutamyl-tRNA amidotransferase subunit C; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362453
• Gene: Gatc Location: Chr5:115471301-115479220 bp, - strand Genetic Position: Chr5, 56.05 cM, cytoband F
• Alliance: Gatc^{tm1a(KOMP)Wtsi} page
• IMPC: Gatc gene page

Mutation origin

• Mutant Cell Lines: EPD0029_1_A05, EPD0029_1_A06, EPD0029_1_B04, EPD0029_1_B05, EPD0029_1_C04, EPD0029_1_C06, EPD0029_1_D04, EPD0029_1_D06, EPD0029_1_E05, EPD0029_1_F04, EPD0029_1_G04, EPD0029_1_G05, EPD0029_1_H05
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 115473257 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 115474052. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Gatc Mutation: 10 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)