Smyd2^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Smyd2^{tm1a(KOMP)Wtsi}
• Name: SET and MYND domain containing 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362468
• Gene: Smyd2 Location: Chr1:189612689-189654560 bp, - strand Genetic Position: Chr1, 95.03 cM, cytoband H6
• Alliance: Smyd2^{tm1a(KOMP)Wtsi} page
• IMPC: Smyd2 gene page

Mutation origin

• Mutant Cell Lines: EPD0028_1_A01, EPD0028_1_A02, EPD0028_1_B03, EPD0028_1_C03, EPD0028_1_D01, EPD0028_1_D03, EPD0028_1_E02, EPD0028_1_F01, EPD0028_1_F02, EPD0028_1_G03
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 189641827 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 189642549. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 2 lines available
• Carrying any Smyd2 Mutation: 138 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 8 reference(s)