Mtfmt^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mtfmt^{tm1a(KOMP)Wtsi}
• Name: mitochondrial methionyl-tRNA formyltransferase; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362485
• Gene: Mtfmt Location: Chr9:65343064-65360336 bp, + strand Genetic Position: Chr9, 35.31 cM, cytoband D
• Alliance: Mtfmt^{tm1a(KOMP)Wtsi} page
• IMPC: Mtfmt gene page

Mutation origin

• Mutant Cell Lines: EPD0042_1_A04, EPD0042_1_A05, EPD0042_1_A05, EPD0042_1_A06, EPD0042_1_C06, EPD0042_1_G04, EPD0042_1_G06, EPD0067_1_A11, EPD0067_1_A12, EPD0067_1_C10, EPD0067_1_C11, EPD0067_1_C12, EPD0067_1_D10, EPD0067_1_D11, EPD0067_1_D12, EPD0067_1_E11, EPD0067_1_E12, EPD0067_1_F10, EPD0067_1_G10, EPD0067_1_G11, EPD0067_1_H12
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 65347199 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 65347986. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:239776)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mtfmt Mutation: 18 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)