Akap9^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Akap9^{tm1a(KOMP)Wtsi}
• Name: A kinase anchor protein 9; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362642
• Synonyms: Akap9^Gt
• Gene: Akap9 Location: Chr5:3977410-4130204 bp, + strand Genetic Position: Chr5, 2.26 cM, cytoband A2
• Alliance: Akap9^{tm1a(KOMP)Wtsi} page
• IMPC: Akap9 gene page

Mutation origin

• Mutant Cell Lines: EPD0033_5_A02, EPD0033_5_A03, EPD0033_5_B01, EPD0033_5_B02, EPD0033_5_B03, EPD0033_5_C01, EPD0033_5_C02, EPD0033_5_C03, EPD0033_5_D01, EPD0033_5_D02, EPD0033_5_D03, EPD0033_5_E02, EPD0033_5_E03, EPD0033_5_F01, EPD0033_5_F02, EPD0033_5_G02, EPD0033_5_G03, EPD0033_5_H01, EPD0033_5_H02, EPD0033_5_H03
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 4018123 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 4019078. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Akap9 Mutation: 181 strains or lines available

Notes

Cell line EPD0033_5_D02 was successfully used to make chimeric mice. Germline transmission was accomplished. J:198499

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 10 reference(s)