Coq9^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Coq9^{tm1a(KOMP)Wtsi}
• Name: coenzyme Q9; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362815
• Synonyms: Coq9^Q95X
• Gene: Coq9 Location: Chr8:95565041-95581523 bp, + strand Genetic Position: Chr8, 46.89 cM
• Alliance: Coq9^{tm1a(KOMP)Wtsi} page
• IMPC: Coq9 gene page

Mutation origin

• Mutant Cell Lines: EPD0112_2_A09, EPD0112_2_B09, EPD0112_2_B11, EPD0112_2_B12, EPD0112_2_C09, EPD0112_2_C10, EPD0112_2_C11, EPD0112_2_D09, EPD0112_2_D10, EPD0112_2_D12, EPD0112_2_E09, EPD0112_2_E10, EPD0112_2_E11, EPD0112_2_F09, EPD0112_2_F10, EPD0112_2_F11, EPD0112_2_F12, EPD0112_2_G09, EPD0112_2_G10, EPD0112_2_G12, EPD0112_2_H09, EPD0112_2_H10, EPD0112_2_H11
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 95570946 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 95571824. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Coq9 Mutation: 27 strains or lines available

Notes

Cell line EPD0112_2_A09 was successfully used to make chimeric mice. Germline transmission was accomplished. J:229185

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 7 reference(s)