Mysm1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mysm1^{tm1a(KOMP)Wtsi}
• Name: myb-like, SWIRM and MPN domains 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362924
• Synonyms: Mysm1tm1a
• Gene: Mysm1 Location: Chr4:94830277-94867337 bp, - strand Genetic Position: Chr4, 43.34 cM
• Alliance: Mysm1^{tm1a(KOMP)Wtsi} page
• IMPC: Mysm1 gene page

Mutation origin

• Mutant Cell Lines: EPD0019_1_A04, EPD0019_1_A05, EPD0019_1_A06, EPD0019_1_B06, EPD0019_1_C04, EPD0019_1_D04, EPD0019_1_D05, EPD0019_1_F04, EPD0019_1_F05, EPD0019_1_F06, EPD0019_1_G05, EPD0019_1_H04, EPD0019_1_H05, EPD0019_1_H06
• Germline Transmission: Earliest citation of germline transmission: J:167648
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_st0
• Mutation details: The L1L2_st0 cassette was inserted at position 94860893 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 94861685. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Mysm1 Mutation: 44 strains or lines available

Notes

EPD0019_1_A04 ES cells were used to generate the mice used in J:179287.
EPD0019_1_A05 ES cells were used to generate the mice used in J:181727.

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 21 reference(s)