Rnf31^{tm2e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnf31^{tm2e(KOMP)Wtsi}
• Name: ring finger protein 31; targeted mutation 2e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363183
• Gene: Rnf31 Location: Chr14:55829199-55841131 bp, + strand Genetic Position: Chr14, 28.19 cM
• IMPC: Rnf31 gene page

Mutation origin

• Mutant Cell Lines: EPD0161_5_B05, EPD0161_5_C05, EPD0161_5_D05
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55831290 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf31 Mutation: 50 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)