Bpnt2^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Bpnt2^{tm1a(KOMP)Wtsi}
• Name: 3'(2'), 5'-bisphosphate nucleotidase 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363750
• Gene: Bpnt2 Location: Chr4:4762484-4793306 bp, - strand Genetic Position: Chr4, 2.56 cM
• Alliance: Bpnt2^{tm1a(KOMP)Wtsi} page
• IMPC: Bpnt2 gene page

Mutation origin

• Mutant Cell Lines: EPD0016_3_C08, EPD0016_3_C09, EPD0016_3_D09, EPD0017_3_B02, EPD0017_3_B03, EPD0017_3_C03, EPD0017_3_D02, EPD0017_3_D03, EPD0017_3_E02, EPD0017_3_F01, EPD0017_3_F03, EPD0017_3_G01, EPD0017_3_G02, EPD0017_3_H01, EPD0017_3_H02, EPD0017_3_H03
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_st0
• Mutation details: The L1L2_st0 cassette was inserted at position 4777929 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 4777929. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Bpnt2 Mutation: 18 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 6 reference(s)