Gm5544^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gm5544^{tm1a(KOMP)Wtsi}
• Name: predicted gene 5544; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4364386
• Gene: Gm5544 Location: Chr3:97837489-97874334 bp, + strand Genetic Position: Chr3, 42.36 cM
• Alliance: Gm5544^{tm1a(KOMP)Wtsi} page
• IMPC: Gm5544 gene page

Mutation origin

• Mutant Cell Lines: EPD0164_6_A12, EPD0164_6_B09, EPD0164_6_D10, EPD0164_6_D11, EPD0164_6_D12, EPD0164_6_E12, EPD0164_6_F11, EPD0164_6_H09
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 97873199 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 97874584. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Gm5544 Mutation: 4 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 7 reference(s)