Id1^tm1Bene
Targeted Allele Detail

Summary

• Symbol: Id1^tm1Bene
• Name: inhibitor of DNA binding 1, HLH protein; targeted mutation 1, Robert Benezra
• MGI ID: MGI:4366905
• Synonyms: Id1^V, Id1^VenusYFP
• Gene: Id1 Location: Chr2:152578171-152579330 bp, + strand Genetic Position: Chr2, 75.41 cM
• Alliance: Id1^tm1Bene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154023
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The coding sequence of the Venus yellow fluorescent protein was linked to the 3' end of the Id1 coding sequence with a sequence for a 10-amino acid flexible linker (GSAGSAAGSG) to create an Id1/YFP fusion protein. Following this insertion, the endogenous 3' untranslated region was unperturbed and utilized as the polyadenylation sequence. The FRT-Neo-FRT cassette for positive selection was inserted 477 bp downstream of the modified Id1 gene. CY2.4 ES cells (albino C57BL/6 derivation) were used for targeting. Southern blot positive ESC clones were injected into co-isogenic B6J blastocysts. Positive chimeras were bred to albino B6J females. Albino pups were genotyped by PCR, and positive pups were bred to B6 ACTB::FLPE mice (Artemis Pharamaceuticals) to excise the Neo cassette in vivo. (J:154023)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Id1 Mutation: 10 strains or lines available

Notes

This mutant allele can substitute for the wild-type allele (i.e. mice that are of Id1(Venus/Venus); Id3(-/-) genotype were viable and normal at weaning).

References

• Original: J:154023 Nam HS, et al., High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009 Nov 6;5(5):515-26
• All: 7 reference(s)