Dynll1^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dynll1^{tm1(KOMP)Wtsi}
• Name: dynein light chain LC8-type 1; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419330
• Gene: Dynll1 Location: Chr5:115435129-115438995 bp, - strand Genetic Position: Chr5, 56.04 cM, cytoband F
• Alliance: Dynll1^{tm1(KOMP)Wtsi} page
• IMPC: Dynll1 gene page

Mutation origin

• Mutant Cell Lines: EPD0397_1_A06, EPD0397_1_B02, EPD0397_1_B09, EPD0397_1_C02, EPD0397_1_C03, EPD0397_1_C05, EPD0397_1_C06, EPD0397_1_C07, EPD0397_1_D02, EPD0397_1_D06, EPD0397_1_D08, EPD0397_1_D12, EPD0397_1_E02, EPD0397_1_E03, EPD0397_1_E06, EPD0397_1_E10, EPD0397_1_E11, EPD0397_1_E12, EPD0397_1_F03, EPD0397_1_F04, EPD0397_1_F09, EPD0397_1_F10, EPD0397_1_F12, EPD0397_1_G02, EPD0397_1_G04, EPD0397_1_G06, EPD0397_1_G07, EPD0397_1_G09, EPD0397_1_G11, EPD0397_1_H01, EPD0397_1_H09, EPD0397_1_H10, EPD0397_1_H11, EPD0397_1_H12
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2296 starting at position 115436415 and ending at position 115438711 of Chromosome 5 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Dynll1 Mutation: 20 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)