Bud31^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Bud31^{tm1(KOMP)Wtsi}
• Name: BUD31 homolog; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419355
• Gene: Bud31 Location: Chr5:145077186-145084888 bp, + strand Genetic Position: Chr5, 85.05 cM
• IMPC: Bud31 gene page

Mutation origin

• Mutant Cell Lines: EPD0407_5_A05, EPD0407_5_A06, EPD0407_5_B06, EPD0407_5_B07, EPD0407_5_C06, EPD0407_5_C07, EPD0407_5_C08, EPD0407_5_D05, EPD0407_5_D08, EPD0407_5_E05, EPD0407_5_E06, EPD0407_5_E08, EPD0407_5_F06, EPD0407_5_F07, EPD0407_5_G06, EPD0407_5_G07, EPD0407_5_G08, EPD0407_5_H05, EPD0407_5_H06, EPD0407_5_H07, EPD0407_5_H08
• Germline Transmission: Unknown
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 4246 starting at position 145079188 and ending at position 145083434 of Chromosome 5 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Bud31 Mutation: 18 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)