Tmem127^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem127^{tm1(KOMP)Wtsi}
• Name: transmembrane protein 127; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419725
• Gene: Tmem127 Location: Chr2:127089868-127103028 bp, + strand Genetic Position: Chr2, 61.87 cM
• Alliance: Tmem127^{tm1(KOMP)Wtsi} page
• IMPC: Tmem127 gene page

Mutation origin

• Mutant Cell Lines: EPD0406_4_A10, EPD0406_4_A11, EPD0406_4_A12, EPD0406_4_B10, EPD0406_4_B11, EPD0406_4_C09, EPD0406_4_C10, EPD0406_4_C11, EPD0406_4_D09, EPD0406_4_E10, EPD0406_4_E12, EPD0406_4_F10, EPD0406_4_F12, EPD0406_4_G09, EPD0406_4_G10, EPD0406_4_G11, EPD0406_4_G12, EPD0406_4_H10, EPD0406_4_H11, EPD0406_4_H12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 1349 starting at position 127097904 and ending at position 127099253 of Chromosome 2 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Tmem127 Mutation: 16 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)