Mta3^{tm3a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mta3^{tm3a(KOMP)Wtsi}
• Name: metastasis associated 3; targeted mutation 3a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419906
• Gene: Mta3 Location: Chr17:84013592-84128945 bp, + strand Genetic Position: Chr17, 54.53 cM, cytoband E4
• Alliance: Mta3^{tm3a(KOMP)Wtsi} page
• IMPC: Mta3 gene page

Mutation origin

• Mutant Cell Lines: EPD0011_3_A08, EPD0011_3_B08, EPD0011_3_C08, EPD0011_3_D08, EPD0011_3_F08, EPD0011_3_G08, EPD0011_3_H08
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: SI6.C21 (ES Cell)
• Strain of Origin: C57BL/6JCrl
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 84045266 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 84046183. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mta3 Mutation: 121 strains or lines available

Notes

Cell line EPD0011_3_B08 was successfully used to make chimeric mice. Germline transmission was accomplished. J:278486

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 6 reference(s)