Tg(Slc1a3-cre/ERT2)45-72Fwp
Transgene Detail

Summary

• Symbol: Tg(Slc1a3-cre/ERT2)45-72Fwp
• Name: transgene insertion 45-72, Frank W Pfrieger
• MGI ID: MGI:4420274
• Synonyms: Glast-CreER^T2, GLAST::CreERT2, Tg(Glast-CreER^T2)45-72
• Transgene: Tg(Slc1a3-cre/ERT2)45-72Fwp Location: unknown
• Alliance: Tg(Slc1a3-cre/ERT2)45-72Fwp page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Slc1a3-cre/ERT2)45-72Fwp expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc1a3 (MGI:99917)

• Mutation details: The creER^T2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Slc1a3 (Glast) gene located in BAC RP23-63O21. The linearized vector was injected into pronuclei of fertilized FVB/N eggs. Line 45-72 showed the highest level of Cre expression, which corresponded to the strong presence of Slc1a3 transcripts. Other regions of the CNS also contained CreER^T2 transcripts, but at lower levels. CreER^T2 mRNA was also detected in the skin and testis in agreement with elevated Slc1a3 transcript levels in these tissues. (J:156087)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc1a3 (mouse) Summary of all recombinase alleles driven by Slc1a3. Comparative matrix view of recombinase activities Alliance mouse Slc1a3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:156087 Slezak M, et al., Transgenic mice for conditional gene manipulation in astroglial cells. Glia. 2007 Nov 15;55(15):1565-76
• All: 20 reference(s)