Lyve1^{tm1.1(EGFP/cre)Cys}
Targeted Allele Detail

Summary

• Symbol: Lyve1^{tm1.1(EGFP/cre)Cys}
• Name: lymphatic vessel endothelial hyaluronan receptor 1; targeted mutation 1, Jason G Cyster
• MGI ID: MGI:4421655
• Synonyms: Lyve-1 Cre⁺, Lyve-1 EGFP-hCre
• Gene: Lyve1 Location: Chr7:110449814-110462160 bp, - strand Genetic Position: Chr7, 57.89 cM, cytoband F2
• Alliance: Lyve1^{tm1.1(EGFP/cre)Cys} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156826
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Lyve1^{tm1.1(EGFP/cre)Cys} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lyve1 (MGI:2136348)

• Mutation details: An EGFP fused to a humanized cre (hCre) sequence and preceded by an internal ribosomal entry site (IRES) was inserted into the 3' UTR of the Lyve1 locus by homologous recombination in E14 ES cells. This construct was followed by an FRT-flanked neo selection cassette. Three successfully targeted clones were injected into C57BL/6 blastocysts and chimeras derived from clone 9 were bred to get germline transmission. The neo cassette was deleted in the germline with Flp recombinase. Selective GFP expression in nuclei of Lyve1 +ve cells is detected by antibody staining. (J:156826)

Generation of the Lyve1^{tm1.1(EGFP/cre)Cys} allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lyve1 (mouse) Summary of all recombinase alleles driven by Lyve1. Comparative matrix view of recombinase activities Alliance mouse Lyve1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lyve1 Mutation: 15 strains or lines available

References

• Original: J:156826 Pham TH, et al., Lymphatic endothelial cell sphingosine kinase activity is required for lymphocyte egress and lymphatic patterning. J Exp Med. 2010 Jan 18;207(1):17-27, S1-4
• All: 65 reference(s)