Cort^tm1(cre)Zjh
Targeted Allele Detail

Summary

• Symbol: Cort^tm1(cre)Zjh
• Name: cortistatin; targeted mutation 1, Z Josh Huang
• MGI ID: MGI:4431363
• Synonyms: Cst-2A-Cre
• Gene: Cort Location: Chr4:149209491-149211132 bp, - strand Genetic Position: Chr4, 78.89 cM
• Alliance: Cort^tm1(cre)Zjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Cort^tm1(cre)Zjh expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cort (MGI:109538)

• Mutation details: A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), a cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cortistatin locus (Cort). This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to generate the colony and remove the neo selection cassette. The FLPe transgene has been bred out of the line. (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cort (mouse) Summary of all recombinase alleles driven by Cort. Comparative matrix view of recombinase activities Alliance mouse Cort gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cort Mutation: 12 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 13 reference(s)