Itm2c^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Itm2c^{tm1a(EUCOMM)Wtsi}
• Name: integral membrane protein 2C; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4431661
• Gene: Itm2c Location: Chr1:85822231-85836419 bp, + strand Genetic Position: Chr1, 43.94 cM
• Alliance: Itm2c^{tm1a(EUCOMM)Wtsi} page
• IMPC: Itm2c gene page

Mutation origin

• Mutant Cell Lines: EPD0351_4_B07, EPD0351_4_C08, EPD0351_4_D06, EPD0351_4_E06, EPD0351_4_E07, EPD0351_4_E08, EPD0351_4_F05, EPD0351_4_F07, EPD0351_4_G07, EPD0351_4_G08
• Germline Transmission: Earliest citation of germline transmission: J:265892
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 85832485 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 85836639. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Itm2c Mutation: 19 strains or lines available

Notes

Regarding clones EDP0351_4_B07 and _F05: No germline transmission occurred with the B07 ES cells, while the F05 ES cells contained a gene duplication at the Itm2c locus and were thus not useful for to generate a loss-of-function allele. (J:265892, PMID:30042348)

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)