Hira^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hira^{tm1a(EUCOMM)Wtsi}
• Name: histone cell cycle regulator; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4431679
• Gene: Hira Location: Chr16:18695787-18789059 bp, + strand Genetic Position: Chr16, 11.69 cM, cytoband A-B1
• Alliance: Hira^{tm1a(EUCOMM)Wtsi} page
• IMPC: Hira gene page

Mutation origin

• Mutant Cell Lines: EPD0181_1_A01, EPD0181_1_A03, EPD0181_1_A04, EPD0181_1_A05, EPD0181_1_A06, EPD0181_1_A07, EPD0181_1_A10, EPD0181_1_A11, EPD0181_1_A12, EPD0181_1_B01, EPD0181_1_B03, EPD0181_1_B04, EPD0181_1_B05, EPD0181_1_B08, EPD0181_1_B10, EPD0181_1_B12, EPD0181_1_C01, EPD0181_1_C02, EPD0181_1_C03, EPD0181_1_C07, EPD0181_1_C08, EPD0181_1_C11, EPD0181_1_D06, EPD0181_1_D09, EPD0181_1_D12, EPD0181_1_E01, EPD0181_1_E05, EPD0181_1_E06, EPD0181_1_E07, EPD0181_1_E08, EPD0181_1_E09, EPD0181_1_E12, EPD0181_1_F03, EPD0181_1_F04, EPD0181_1_F05, EPD0181_1_F06, EPD0181_1_F08, EPD0181_1_F09, EPD0181_1_F11, EPD0181_1_F12, EPD0181_1_G03, EPD0181_1_G04, EPD0181_1_G06, EPD0181_1_G12, EPD0181_1_H02, EPD0181_1_H05, EPD0181_1_H06, EPD0181_1_H08, EPD0181_1_H10, EPD0181_1_H12
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 18715909 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 18716842. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Hira Mutation: 74 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 12 reference(s)