Smoc1^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Smoc1^{tm1a(EUCOMM)Wtsi}
• Name: SPARC related modular calcium binding 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4431709
• Synonyms: Smoc1^tm1a
• Gene: Smoc1 Location: Chr12:81073582-81233188 bp, + strand Genetic Position: Chr12, 37.28 cM
• Alliance: Smoc1^{tm1a(EUCOMM)Wtsi} page
• IMPC: Smoc1 gene page

Ophthalmo-acromelic-like phenotype in Smoc1^{tm1a(EUCOMM)Wtsi}/Smoc1^{tm1a(EUCOMM)Wtsi} mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0348_1_A01, EPD0348_1_B01, EPD0348_1_C02, EPD0348_1_C03, EPD0348_1_D01, EPD0348_1_E01, EPD0348_1_E02, EPD0348_1_F01, EPD0348_1_F03, EPD0348_1_F04, EPD0348_1_G02, EPD0348_1_H02, EPD0348_1_H03
• Germline Transmission: Earliest citation of germline transmission: J:174198
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 81182092 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 81182939. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Generation of the Smoc1^tm1a(EUCOMM)Wtsi allele

Disease models

Expression

In Mice Carrying this Mutation:	19 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Smoc1 Mutation: 33 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 6 reference(s)