Eed^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Eed^{tm1a(EUCOMM)Wtsi}
• Name: embryonic ectoderm development; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4431778
• Gene: Eed Location: Chr7:89603862-89630184 bp, - strand Genetic Position: Chr7, 50.42 cM
• Alliance: Eed^{tm1a(EUCOMM)Wtsi} page
• IMPC: Eed gene page

Mutation origin

• Mutant Cell Lines: EPD0103_4_A05, EPD0103_4_B04, EPD0103_4_B05, EPD0103_4_B06, EPD0103_4_C04, EPD0103_4_C05, EPD0103_4_C06, EPD0103_4_D04, EPD0103_4_D06, EPD0103_4_F04, EPD0103_4_G04, EPD0103_4_G06, EPD0103_4_H05
• Germline Transmission: Earliest citation of germline transmission: J:213769
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 89616630 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 89617432. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Eed Mutation: 116 strains or lines available

Notes

Cell line EPD0103_4_F04 was successfully used to make chimeric mice. Germline transmission was accomplished. J:213769

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 4 reference(s)