Rhot1^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rhot1^{tm1a(EUCOMM)Wtsi}
• Name: ras homolog family member T1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432006
• Synonyms: Miro1^KO
• Gene: Rhot1 Location: Chr11:80099845-80158733 bp, + strand Genetic Position: Chr11, 47.62 cM, cytoband B5
• Alliance: Rhot1^{tm1a(EUCOMM)Wtsi} page
• IMPC: Rhot1 gene page

Mutation origin

• Mutant Cell Lines: EPD0066_2_A01, EPD0066_2_A02, EPD0066_2_A03, EPD0066_2_B01, EPD0066_2_B02, EPD0066_2_B03, EPD0066_2_C01, EPD0066_2_C02, EPD0066_2_C03, EPD0066_2_D01, EPD0066_2_D03, EPD0066_2_E01, EPD0066_2_E02, EPD0066_2_F01, EPD0066_2_F02, EPD0066_2_F03, EPD0066_2_G01, EPD0066_2_G02, EPD0066_2_G03, EPD0066_2_H01, EPD0066_2_H02, EPD0066_2_H03
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 80110547 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 80111572. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Mice Carrying this Mutation:

30 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Rhot1 Mutation: 44 strains or lines available

Notes

Cell line EPD0066_2_F01 was successfully used to make chimeric mice. Germline transmission was accomplished. J:240500

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 11 reference(s)