Mad2l2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mad2l2^{tm1a(EUCOMM)Wtsi}
• Name: MAD2 mitotic arrest deficient-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432091
• Gene: Mad2l2 Location: Chr4:148214841-148230156 bp, + strand Genetic Position: Chr4, 78.67 cM, cytoband E1
• Alliance: Mad2l2^{tm1a(EUCOMM)Wtsi} page
• IMPC: Mad2l2 gene page

Mutation origin

• Mutant Cell Lines: EPD0046_3_A02, EPD0046_3_A03, EPD0046_3_B01, EPD0046_3_B02, EPD0046_3_B03, EPD0046_3_C02, EPD0046_3_C03, EPD0046_3_D02, EPD0046_3_D03, EPD0046_3_E01, EPD0046_3_E03, EPD0046_3_F01, EPD0046_3_F02, EPD0046_3_F03, EPD0046_3_G01, EPD0046_3_G02, EPD0046_3_G03, EPD0046_3_H01, EPD0046_3_H02, EPD0046_3_H03
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 148227736 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 148228385. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Mad2l2 Mutation: 15 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 7 reference(s)