Chst5^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Chst5^{tm1a(EUCOMM)Wtsi}
• Name: carbohydrate sulfotransferase 5; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432310
• Gene: Chst5 Location: Chr8:112615767-112636831 bp, - strand Genetic Position: Chr8, 58.11 cM, cytoband E1
• Alliance: Chst5^{tm1a(EUCOMM)Wtsi} page
• IMPC: Chst5 gene page

Mutation origin

• Mutant Cell Lines: EPD0430_4_A02, EPD0430_4_A04, EPD0430_4_B01, EPD0430_4_B02, EPD0430_4_C02, EPD0430_4_C03, EPD0430_4_C04, EPD0430_4_D01, EPD0430_4_D02, EPD0430_4_D03, EPD0430_4_D04, EPD0430_4_E01, EPD0430_4_E02, EPD0430_4_E03, EPD0430_4_F01, EPD0430_4_F02, EPD0430_4_G01, EPD0430_4_G02, EPD0430_4_G03, EPD0430_4_H01, EPD0430_4_H03
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 112615360 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 112618082. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Chst5 Mutation: 76 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)