Fto^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Fto^{tm1a(EUCOMM)Wtsi}
• Name: FTO alpha-ketoglutarate dependent dioxygenase; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432397
• Synonyms: Fto^fl, FTO^flox
• Gene: Fto Location: Chr8:92040153-92395067 bp, + strand Genetic Position: Chr8, 44.34 cM
• Alliance: Fto^{tm1a(EUCOMM)Wtsi} page
• IMPC: Fto gene page

Mutation origin

• Mutant Cell Lines: EPD0103_5_A10, EPD0103_5_A11, EPD0103_5_A12, EPD0103_5_B11, EPD0103_5_C10, EPD0103_5_D10, EPD0103_5_D11, EPD0103_5_E10, EPD0103_5_E11, EPD0103_5_E12, EPD0103_5_F10, EPD0103_5_F11, EPD0103_5_G10, EPD0103_5_G11, EPD0103_5_H10, EPD0103_5_H11, EPD0103_5_H12
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 92135427 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 92137108. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:203742)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Fto Mutation: 75 strains or lines available

Notes

Cell line EPD0103_5_A10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:228836

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 11 reference(s)