Arid4a^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Arid4a^{tm1a(EUCOMM)Wtsi}
• Name: AT-rich interaction domain 4A; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432398
• Gene: Arid4a Location: Chr12:71062764-71145366 bp, + strand Genetic Position: Chr12, 29.39 cM
• Alliance: Arid4a^{tm1a(EUCOMM)Wtsi} page
• IMPC: Arid4a gene page

Mutation origin

• Mutant Cell Lines: EPD0027_5_A08, EPD0027_5_B07, EPD0027_5_B09, EPD0027_5_C07, EPD0027_5_C08, EPD0027_5_C09, EPD0027_5_D07, EPD0027_5_D08, EPD0027_5_D09, EPD0027_5_E07, EPD0027_5_E08, EPD0027_5_E09, EPD0027_5_F07, EPD0027_5_F08, EPD0027_5_F09, EPD0027_5_G07, EPD0027_5_G09, EPD0027_5_H08, EPD0027_5_H09
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 71107615 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 71108645. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Arid4a Mutation: 60 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 6 reference(s)