Ocm^{tm1e(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ocm^{tm1e(EUCOMM)Wtsi}
• Name: oncomodulin; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432497
• Gene: Ocm Location: Chr5:143956622-143963488 bp, - strand Genetic Position: Chr5, 82.85 cM, cytoband G1-3
• Alliance: Ocm^{tm1e(EUCOMM)Wtsi} page
• IMPC: Ocm gene page

Mutation origin

• Mutant Cell Lines: EPD0397_5_A04, EPD0397_5_A06, EPD0397_5_B03, EPD0397_5_B08, EPD0397_5_B09, EPD0397_5_B10, EPD0397_5_C01, EPD0397_5_C03, EPD0397_5_C06, EPD0397_5_C08, EPD0397_5_D08, EPD0397_5_D10, EPD0397_5_E02, EPD0397_5_E04, EPD0397_5_E10, EPD0397_5_F05, EPD0397_5_F07, EPD0397_5_F10, EPD0397_5_F11, EPD0397_5_G01, EPD0397_5_G04, EPD0397_5_G06, EPD0397_5_G08, EPD0397_5_G09, EPD0397_5_G10, EPD0397_5_H04, EPD0397_5_H05, EPD0397_5_H06, EPD0397_5_H07, EPD0397_5_H09, EPD0397_5_H10, EPD0397_5_H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 143961823 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Ocm Mutation: 9 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)