Slx4^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slx4^{tm1a(EUCOMM)Wtsi}
• Name: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae); targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4432843
• Synonyms: Btbd12^f3, Btbd12^{tm1a(EUCOMM)Wtsi}, Fancp^-
• Gene: Slx4 Location: Chr16:3796969-3821634 bp, - strand Genetic Position: Chr16, 2.34 cM
• Alliance: Slx4^{tm1a(EUCOMM)Wtsi} page
• IMPC: Slx4 gene page

Mutation origin

• Mutant Cell Lines: EPD0028_7_A07, EPD0028_7_A08, EPD0028_7_A09, EPD0028_7_E07, EPD0028_7_E09, EPD0028_7_F08, EPD0028_7_H09
• Germline Transmission: Earliest citation of germline transmission: J:168567
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 3813295 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 3814186. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:168567, J:173534)

Expression

In Structures Affected by this Mutation:

15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Slx4 Mutation: 57 strains or lines available

Notes

J:168567 does not specify mutant cell line used. J:173448 made mice from the mutant cell line EPD0028_7_A08

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 11 reference(s)