Eef2kmt^{tm1e(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Eef2kmt^{tm1e(EUCOMM)Wtsi}
• Name: eukaryotic elongation factor 2 lysine methyltransferase; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433018
• Gene: Eef2kmt Location: Chr16:5062019-5073820 bp, - strand Genetic Position: Chr16, 2.51 cM, cytoband A2
• Alliance: Eef2kmt^{tm1e(EUCOMM)Wtsi} page
• IMPC: Eef2kmt gene page

Mutation origin

• Mutant Cell Lines: EPD0146_4_A05, EPD0146_4_A06, EPD0146_4_D05, EPD0146_4_D06, EPD0146_4_E08, EPD0146_4_F05, EPD0146_4_F06, EPD0146_4_G05, EPD0146_4_G06, EPD0146_4_G08, EPD0146_4_H06
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 5067802 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Eef2kmt Mutation: 55 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)