Raf1^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Raf1^{tm1a(EUCOMM)Wtsi}
• Name: v-raf-leukemia viral oncogene 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433410
• Gene: Raf1 Location: Chr6:115595530-115653596 bp, - strand Genetic Position: Chr6, 53.62 cM, cytoband C3
• Alliance: Raf1^{tm1a(EUCOMM)Wtsi} page
• IMPC: Raf1 gene page

Mutation origin

• Mutant Cell Lines: EPD0065_4_A07, EPD0065_4_B07, EPD0065_4_B09, EPD0065_4_C07, EPD0065_4_C08, EPD0065_4_D07, EPD0065_4_D08, EPD0065_4_D09, EPD0065_4_E08, EPD0065_4_F08, EPD0065_4_G07
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 115602969 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 115604387. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Raf1 Mutation: 117 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)