Cdc14b^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cdc14b^{tm1a(EUCOMM)Wtsi}
• Name: CDC14 cell division cycle 14B; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433546
• Gene: Cdc14b Location: Chr13:64337082-64423104 bp, - strand Genetic Position: Chr13, 33.26 cM
• IMPC: Cdc14b gene page

Mutation origin

• Mutant Cell Lines: EPD0338_2_A02, EPD0338_2_A03, EPD0338_2_A04, EPD0338_2_B02, EPD0338_2_C02, EPD0338_2_D01, EPD0338_2_D02, EPD0338_2_E01
• Germline Transmission: Unknown
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 64373145 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 64374146. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Cdc14b Mutation: 38 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)